Fig. 1. Effect of the absence of TRPC6 on litter size and placental markers. A: The number of pups was systematically counted at post-natal day 1 (first day of birth). On average the litter size of C57Bl6/J, B6129SF2/J and TRPC6^-/- mice (with n=13, 10 and 16 gestations, respectively). * p<0.05 (one-way ANOVA followed by Bonferroni's test) vs C57Bl6/J and vs B6129SF2/J mice. B: The relative abundance of genes involved in placenta development and functions (proliferin, Gcm1, Mash2, CD31) was analyzed by real-time RT-qPCR in the 3 groups of mice (C57Bl6/J, B6129SF2/J and TRPC6^-/-). Data are normalized to C57Bl6/J mice with n= 4 per group. Mean ± SEM with * p<0.05 (one-way ANOVA followed by Bonferroni's test) vs C57Bl6/J and vs B6129SF2/J mice.